10X WTB Buffer (Western Blot Transfer Buffer) Solution pH 8.3 500ml

SKU : L-1256.0500
CAS : Not applicable
Formula : Not applicable
Grade : For Molecular Biology /Electrophoresis Grade

A 10X WTB (Western Blot Transfer) Buffer at pH 8.3 is a concentrated solution used for transferring proteins from polyacrylamide gels to a membrane, typically nitrocellulose or PVDF, during a Western blot. It is diluted to a 1X working solution with water and methanol before use, creating a standard Tris-Glycine buffer that facilitates protein migration from the gel to the membrane for subsequent antibody detection.

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A “10X WTB Buffer (Western Blot Transfer Buffer) Solution pH 8.3” is a concentrated stock solution used in Western blotting to transfer proteins from a gel to a membrane. The “10X” means it must be diluted 10-fold to create a working “1X” buffer solution, and the pH of 8.3 is a key component for protein migration. The buffer typically contains Tris and glycine, often with methanol to enhance protein binding to the membrane.

TINDAKAN PENCEGAHAN UNTUK PENANGANAN YANG AMAN

Hindari kontak dengan kulit dan mata. Hindari menghirup uap atau kabut. Untuk tindakan pencegahan, lihat bagian 2.2 dalam SDS.

 

KONDISI PENYIMPANAN YANG AMAN, TERMASUK KETIDAKCOCOKAN

a. Kondisi penyimpanan

Simpan di tempat yang sejuk. Tutup wadah rapat-rapat di tempat yang kering dan berventilasi baik.

Suhu penyimpanan : Rekomendasi suhu penyimpanan lihat pada label

b. Kelas penyimpanan

Kelas penyimpanan (TRGS 510): Cairan Tidak Mudah Terbakar

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