10X WTB Buffer (Western Blot Transfer Buffer) Solution pH 8.3 500ml

SKU : L-1256.0500

CAS : Not applicable

Formula : Not applicable

Grade : For Molecular Biology /Electrophoresis Grade

A 10X WTB (Western Blot Transfer) Buffer at pH 8.3 is a concentrated solution used for transferring proteins from polyacrylamide gels to a membrane, typically nitrocellulose or PVDF, during a Western blot. It is diluted to a 1X working solution with water and methanol before use, creating a standard Tris-Glycine buffer that facilitates protein migration from the gel to the membrane for subsequent antibody detection.

A “10X WTB Buffer (Western Blot Transfer Buffer) Solution pH 8.3” is a concentrated stock solution used in Western blotting to transfer proteins from a gel to a membrane. The “10X” means it must be diluted 10-fold to create a working “1X” buffer solution, and the pH of 8.3 is a key component for protein migration. The buffer typically contains Tris and glycine, often with methanol to enhance protein binding to the membrane.

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