10X PBST Buffer (Phosphate Buffered Saline with Tween 20) Solution pH 7.4 500ml

SKU : L-1260.0500

CAS : Not applicable

Formula : Not applicable

Grade : For Molecular Biology /Electrophoresis Grade

10X PBST buffer is primarily used as a wash buffer in immunoassays like ELISA and Western blotting to wash away unbound antibodies and reduce non-specific binding. Its other uses include cell culture, general protein research, and as a component in other biological assays where maintaining a stable pH and reducing background noise is important. The 10X concentrate must be diluted with distilled water to create a 1X working solution for experiments.

A 10X PBST buffer is a concentrated stock solution of Phosphate Buffered Saline that includes the non-ionic surfactant Tween-20. This solution is used primarily as a wash buffer in immunoassays and related biological procedures after dilution to its 1X working concentration.

Explanation

The 10X PBST solution is a specialized variant of standard phosphate buffered saline (PBS) with the addition of Tween-20, a non-ionic detergent.

10X Concentration: The “10X” indicates that the solution is ten times more concentrated than the final “1X” working concentration. It must be diluted with distilled or deionized water (typically 1 part 10X PBST to 9 parts water) before use in experiments.
Function: The primary role of the buffer is to maintain a stable physiological pH (around 7.4 when diluted) and osmotic strength, which helps preserve the integrity of biological samples. The inclusion of Tween-20 minimizes non-specific binding of proteins (e.g., antibodies) to membranes or microtiter plate wells, thereby reducing background noise and improving the specific signal in assays.
Common Applications: PBST is widely used as a wash buffer in procedures such as:
- Western blotting
- ELISA (Enzyme-Linked Immunosorbent Assay)
- Immunoprecipitation

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