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Key Applications
Microbial Screening: Rapid screening and quantification of IAA-producing bacteria, fungi, and actinomycetes in rhizospheric soil samples.
Plant Physiology Research: Monitoring auxin biosynthesis pathways and hormone distribution within plant organs.
Agricultural Quality Control: Assessing the bioactivity and efficacy of microbial inoculants or biofertilizers.
Features and Benefits
High Sensitivity: Capable of detecting microgram levels (µg/mL) of IAA in culture supernatants or plant extracts.
Spectrophotometric Compatibility: The developed pink complex exhibits stable absorbance, typically measured quantitatively at 530 nm
Ready-to-Use Formulation: Prepared under strict laboratory standards to ensure batch-to-batch consistency and reliable reproducible results.
Note: This specific formulation containing FeCl3 is designed exclusively for indole/auxin analysis and is not suitable for the Salkowski cholesterol (sterol) test.
Salkowski Reagent is a specialized colorimetric indicator formulated primarily for the qualitative detection and quantitative estimation of Indole-3-acetic acid (IAA), the principal plant hormone of the auxin class. Widely utilized in plant physiology, agricultural biotechnology, and soil microbiology, this reagent serves as an essential tool for identifying auxin-producing plant growth-promoting rhizobacteria (PGPR) and analyzing endogenous auxin levels in plant tissues.
Chemical Composition & Specification
Unlike the classic Salkowski variant used for sterol analysis, this formulation is optimized for indole detection. It contains precisely measured concentrations of Iron (III) Chloride (FeCl3) dissolved in an acidic matrix (typically optimized with Perchloric Acid (HClO4) or Sulfuric Acid (H2SO4). The inclusion of ferric ions (Fe3+) acts as a highly specific oxidizing agent tailored to react with the indole ring structure.
Principle of the Assay
The assay relies on a specific colorimetric reaction where the Fe3+ ions complex with and oxidize the indole-3-acetic acid molecule under strongly acidic conditions. This chemical interaction induces a distinct chromatic shift, transforming the solution from a faint yellowish tint to a vibrant pink to reddish-pink color. The color intensity directly correlates with the concentration of IAA present in the sample.
TINDAKAN PENCEGAHAN UNTUK PENANGANAN YANG AMAN
a. Saran tentang penanganan yang aman
Bekerja di bawah sungkup. Jangan menghirup zat/campuran. Hindari pembentukan uap/aerosol.
b. Saran tentang perlindungan terhadap kebakaran dan ledakan
Jauhkan dari api terbuka, permukaan panas, dan sumber penyulutan.
c. Tindakan kebersihan
Segera ganti pakaian yang terkontaminasi. Gunakan pelindung kulit preventif. Cuci tangan dan wajah setelah bekerja dengan zat tersebut.
KONDISI PENYIMPANAN YANG AMAN, TERMASUK KETIDAKCOCOKAN
a. Kondisi penyimpanan
Tidak boleh menggunakan wadah logam. Tertutup rapat. Hanya disimpan secara terpisah atau bersama dengan zat pengoksidasi lainnya dan jauh dari sumber penyulutan dan panas. Karena potensi oksidasinya, produk ini dapat meningkatkan laju pembakaran zat yang mudah terbakar secara substansial atau menyulut zat yang mudah terbakar saat bersentuhan dengannya.
Suhu penyimpanan : Rekomendasi suhu penyimpanan lihat pada label
b. Kelas penyimpanan
Kelas penyimpanan (TRGS 510): 5.1A: Bahan berbahaya yang sangat oksidatif
Your orders are shipped seamlessly between countries
Your orders are shipped seamlessly between countries
You have the right to return your orders within 30 days.
0251-8359110 (Call)
+62 813 1689 4650 (WA)
+62 8953 6506 8181 (WA)
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