10X Tris-MES Buffer Solution pH 7.3 500ml

SKU : L-1258.0500

CAS : Not applicable

Formula : Not applicable

Grade : For Molecular Biology /Electrophoresis Grade

10X Tris-MES buffer solution with a pH of 7.3 is primarily used in protein electrophoresis, specifically with Bis-Tris gels, for separating small- to medium-sized proteins. The 10X concentration is a stock solution that must be diluted to 1X before use.

A 10X Tris-MES buffer solution, pH 7.3, is a concentrated stock solution combining two buffering agents, Tris and MES, designed to maintain a stable pH of approximately 7.3 in biological and biochemical applications, particularly protein electrophoresis. This denotes a 10-fold concentrated stock solution. It is prepared at a higher concentration for convenience of storage and stability. Before use in experiments, it must be diluted with deionized or nuclease-free water to its working concentration (1X). For example, to make 1 liter of 1X solution, you would mix 100 mL of the 10X stock with 900 mL of water.

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