10X TE Buffer (Tris-EDTA) Solution pH 8.0 500ml

SKU : L-1246.0500

CAS : Not applicable

Formula : Not applicable

Grade : for microbiology

10X TE Buffer (Tris-EDTA) Solution at pH 8.0 is a stock solution used to stabilize and protect nucleic acids (DNA/RNA) from degradation during storage, purification, and other molecular biology applications like gel electrophoresis, cloning, and sequencing. Its primary functions are to maintain a stable pH with Tris and inhibit enzymes called nucleases by chelating metal ions with EDTA.

The term “10X TE Buffer (Tris-EDTA) Solution pH 8.0” refers to a concentrated stock solution used primarily in molecular biology that is specifically formulated to protect DNA and RNA from degradation.The “10X” indicates that the solution is a concentrated stock that must be diluted ten times (1:10 ratio) with distilled or deionized water to achieve the working strength, known as “1X” concentration. This saves storage space and preparation time.

10X Concentration: The standard concentration for a 10X TE buffer is typically 100 mM Tris and 10 mM EDTA.
1X (Working) Concentration: After dilution, the ready-to-use 1X solution contains 10 mM Tris and 1 mM EDTA.

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