Acid Chrome Blue K is primarily used as a metal indicator and in staining procedures for biomedical applications. It’s also used in research, particularly in drug development and disease diagnosis for detecting specific proteins and cellular structures. Additionally, it’s used in Loop-mediated isothermal amplification (LAMP) assays to visualize the reaction based on magnesium ion concentration changes.

Applications:

• Metal Indicator:

  Acid Chrome Blue K acts as a metal indicator, changing color based on the concentration of metal ions in a solution. This property is particularly useful in LAMP assays, where the color change indicates the completion of the reaction. 
• Staining Agent:

  It’s used in histology and microscopy for staining various biological samples, allowing for clearer visualization of cells and tissues under a microscope. 
• Research Applications:

  It is used in research, especially in drug development and disease diagnosis, to detect specific proteins and cellular structures. It is also used in the study of dye adsorption and separation. 
• LAMP Assays:

  The color change of Acid Chrome Blue K in LAMP reactions is due to its ability to bind magnesium ions. A decrease in magnesium ion concentration, which occurs during the LAMP reaction, causes a color shift, making it a useful visual indicator of reaction progress.