Immersion Oil For Microscopy 125 mL

SKU : B-3087.0125
CAS : Not Applicable
Formula : Not Applicable
Grade : for Microscopy
Immersion oil improves the resolution and clarity of microscope images by reducing light refraction between the slide and the lens. This allows more light rays to enter the lens, resulting in a brighter, sharper image with greater detail.
Immersion oil is most critical for high magnification, so it’s typically used with objectives that have a short focus and higher power. These objectives are usually between 40x and 120x.

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In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.Without oil, light waves reflect off the slide specimen through the glass cover slip, through the air, and into the microscope lens (see the colored figure to the right). Unless a wave comes out at a 90-degree angle, it bends when it hits a new substance, the amount of bend depending on the angle. This distorts the image. Air has a very different index of refraction from glass, making for a larger bend compared to oil, which has an index more similar to glass. Specially manufactured oil can have nearly exactly the same refractive index as glass, making an oil immersed lens nearly as effective as having glass entirely around the sample (which would be impractical).Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy. Typical oils used have an index of refraction of around 1.515.  An oil immersion objective is an objective lens specially designed to be used in this way. Many condensers also give optimal resolution when the condenser lens is immersed in oil.

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